Based on comprehensive analysis on current situation of urban underground space engineering specialty construction, shortcomings of practical teaching for talents cultivation were pointed out. A new idea to structure innovative talents cultivation mechanism of urban underground space engineering specialty based on school-enterprise cooperation was put forward by discussing the effects of school- enterprise cooperation for high-level innovative composite engineering talents cultivation. Combined with the characteristics of urban underground space engineering specialty and the importance of practical teaching, many aspects of practical teaching system were expounded, such as setting practical teaching principles and schemes according to talent cultivation targets, structuring practical teaching system with the entry point of multiple integration, constructing an assessment and evaluation system by the standards of degree of satisfaction of students and enterprises, and establishing a long term security system driven by mutual benefit and win-win.