It is necessary to reform the experiment teaching in universities if we want to cultivate students' creative thinking, innovation ability, observation ability and team coordination ability. Through the investigation and analysis of the current situation of laboratory using in teaching of building environment and energy application engineering in regional universities, I found out that universities focused on theoretical teaching rather than experimental teaching, the number of laboratory equipment was insufficient, and the location of the teaching laboratory was not reasonable. To cultivate students' spirit of innovation and engineering practice ability, the experiment teaching system of "one-four-four" should be constructed and improved, and effective ways for experimental teaching reform should be put forward as ensuring unchanged total amount of class hours. The practice result shows that it can cultivate students' scientific and technological innovation ability, and improve their engineering practice ability and comprehensive quality.